The flow cytometer Diaries
The flow cytometer Diaries
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Details-enriched characterization of cell populations. Morphological information and facts from photographs can include towards the richness of flow cytometry data. For example, the figure displays an in any other case regular apoptosis assay applying Annexin V and PI, incorporating cell imaging to characterize cells in Every single population to reveal morphologically distinct features. These insights couldn't are actually gained from multiplex staining on your own.
Irrespective of whether you’re a novice or an authority, discover our methods to progress your research and abilities in flow cytometry.
(B) RAW 264.7 macrophages are sometimes annotated inaccurately with respect to particle rely because of their complexity and noncircular borders. The volume of singlets accessible for Assessment raises when the user trains and employs a more correct design.
Uncover new insights into your experimental knowledge by visualizing populations applying blended fluorescence and morphology details. Our application interface wizards help downsampling, concatenation (merged files) and dimensionality applications that happen to be developed in to the software package for an intuitive, streamlined facts Assessment encounter.
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In this article, a highly skilled consumer has gated singlets confidently. Immediately after assessing the guide singlet gate, the CytPix impression-derived parameter ParticleCount reveals this gate has much more than 4% aggregates.
Optimizing gating tactic. Even robust handbook singlet gating is error-inclined and stays a subjective conclusion place in Virtually all flow cytometry assays. Imaging can be utilized to confirm and adjust gates to incorporate only single cells of fascination.
Consistent impression quality even at significant throughput Acoustic focusing in addition to a higher-pace camera Blend to graphic these T cells consistently at low or high flow fees. Simply adjust focus and camera settings to fulfill experimental needs.
The Attune NxT cytometer could be the reliable workhorse instrument your lab can rely on for consistent and trusted efficiency. The Attune CytPix product includes a higher-pace brightfield digital camera and AI-driven image analysis software, with modern updates such as machine Discovering equipment and an intuitive consumer interface, enabling enhanced knowledge precision, accuracy, and more rapidly insights into sample biology.
End users can view celebration pictures in genuine-time to substantiate sample good quality, optimize protocols, and document the morphology of each and every cell populace determined for Assessment. Imaging capture also Rewards from acoustic concentrating, which really helps to situation the cells in order that a pointy, centered impression is obtained.
AI-driven automated image Examination Using the Attune Cytometric Software package translates occasion options into distinctive morphology parameters that can be combined with normal fluorescence and scatter parameters to expose extremely reproducible inhabitants adjustments and exceptional subsets.
Software Observe: Automatic image Evaluation lessens consumer-to-consumer variability in flow cytometry gating methods
Leverage the power of Sophisticated technology and innovation to speed up your discoveries. Rely on inside the Attune Flow Cytometer relatives to provide the trustworthiness, velocity, and precision that established your work aside.
No wash/no lyse leukocyte analysis with imaging. Cells ended up acquired from 24-hour-aged blood by dilution in 1 mM EDTA ( Expert UX/UI enables end users to coach AI types for expanded apps. (A) The “Teach Design” attribute offers annotation resources and authentic-time product education accuracy.
Gating on these prolonged parameters means that you can promptly and properly recognize populations of desire and make sure gating strategy with little if any handbook evaluation. The Attune computer software delivers use of point out-of-the-artwork device Discovering Examination tools within an expertly built person interface, which makes it extremely simple to operate.
The Attune Xenith cytometer is really a large-parameter spectral analyzer, supplying both of those spectral unmixing and standard flow capabilities for complete and flexible cell Examination.
The Attune CytPix Flow Cytometer more info permits buyers to visualize samples for the duration of acquisition, validate the precision of gating strategies, and support final results with dependable documentation for publication needs.
What seemed to be a homogeneous inhabitants is really extra assorted—an insight that needs to be regarded when interpreting outcomes.